The B1 night bus line of the Red Búho (meaning Owl) Network, which links Asunción with San Lorenzo, will implement a route adjustment starting Saturday, 1 November 2025. This change aims to enhance connectivity with vital hubs in Paraguay’s capital, particularly the Asunción Bus Station (commonly referred to as the Terminal) where a significant volume of passenger traffic converges.
Under the new arrangement, services heading from Asunción to San Lorenzo will extend to the Terminal before proceeding along Eusebio Ayala Avenue, and resuming the standard path. The return journey from San Lorenzo to Asunción will remain unaltered.
Optimising night-time connectivity
This modification is designed to improve service coverage and bolster links across various night corridors, especially following the recent addition of new Owl lines such as the B3, which serves Asunción and Ñemby.
Paraguayan’s Vice-Ministry of Transport is driving the initiative to create a more efficient, accessible, and evenly distributed network, with a focus on passenger convenience. The B1 night service will maintain a 40-minute frequency, operating daily from 22:00 to 03:20.
Detailed outbound route from Asunción
Passengers travelling from Asunción to San Lorenzo will board the B1 night bus line at Cristóbal Colón Street in the city centre. The route will then proceed via Oliva, Cerro Corá, Brasil, and España Avenue.
From there, it will continue along General José de San Martín, República Argentina, Fernando de la Mora, and Profesora Sofía Mendoza streets, before turning onto Don Osvaldo Kallsen and rejoining República Argentina. The journey will then follow Doctor Eusebio Ayala, Mariscal Estigarribia (Route PY02), and Agrónomo Avenue, culminating at General Genes Street in San Lorenzo.
Return journey to the capital
On the inbound leg from San Lorenzo, the bus will start at General Genes and travel along Julia Miranda Cueto, Mariscal Estigarribia (Route PY02), Doctor Eusebio Ayala, República Argentina, and General José de San Martín.
It will then pass through Papa Juan XXIII, Profesora Delia Frutos de González, Aviadores del Chaco, and Avenida España, before re-entering the city centre via Estados Unidos, Félix de Azara, Díaz, Hernandarias, República, and Cristóbal Colón streets, completing the overnight service to Asunción.
